Joe Biden said US Donald Trump has "cloaked America in darkness for much too long", as he accepted the Democratic White House nomination.
The former US vice-president said his rival has unleashed "too much anger, too much fear, too much division".
Mr Biden's impassioned speech was the capstone of a political career spanning nearly half a century.
He heads into the general election campaign with a clear lead in opinion polls over Mr Trump, 74.
But with 75 days to go until the election the Republican president has plenty of time to narrow the gap.
Speaking from a mostly empty event centre in his hometown of Wilmington, Delaware, Mr Biden, 77, said: "Here and now, I give you my word, if you entrust me with the presidency, I will draw on the best of us, not the worst.
"I'll be an ally of the light, not the darkness.
"It's time for us, for we the people, to come together and make no mistake, united we can and will overcome this season of darkness in America.
"We'll chose hope over fear, facts over fiction, fairness over privilege."
Mr Biden said "character is on the ballot" this November.
"We can choose a path of becoming angrier, less hopeful, more divided, a path of shadow and suspicion," he said.
"Or, or, we can choose a different path and together take this chance to heal, to reform, to unite. A path of hope and light.
"This is a life-changing election. This will determine what America going to look like for a long, long time."
He continued: "What we know about this president is that if he's given four more years, he'll be what he's been for the last four years.
"A president who takes no responsibility, refuses to lead, blames others, cosies up to dictators and fan the flames of hate and division.
"He'll wake up every day believing the job is all about him, never about you.
"Is that the America you want for you, your family and your children?"
Paraphrasing the Irish poet Seamus Heaney, he concluded: "This is our moment to make hope and history rhyme."
His live speech marked the grand finale of the four-night Democratic convention.
But there was no balloon drop, cheering throngs, or any of the other fanfare and razzamatazz of the typical American party conference, because of the coronavirus pandemic.
Organisers opted instead for a largely virtual set piece of pre-recorded speeches crunched into two hours of highly produced programming each evening.
Thursday night's climax was hosted by actress Julia Louis-Dreyfus, the star of US political satire Veep and a vocal critic of Mr Trump.
Speakers at the convention over the past three nights have depicted Mr Trump as incompetent, selfish and a danger to democracy, imploring Americans to vote him out of office – a tone which Mr Biden heavily echoed.
On Wednesday his running mate, California Senator Kamala Harris, became the first black woman to accept a major party's vice-presidential nomination.
The daughter of Indian and Jamaican immigrants assailed Mr Trump's "failure of leadership" and amplified the cries for racial justice that have divided the nation.
"There is no vaccine for racism. We have got to do the work," she said, adding: "None of us are free until all of us are free."
Call it Joe Biden's "return to normalcy" speech.
That was Warren G Harding campaign slogan when he ran for president in 1920, with a campaign centred around healing and calming Americans after the trauma of World War One.
In his winning presidential bid, he preached healing, serenity and restoration. To put it in modern terms, an end to all the drama.
Mr Biden bills his campaign as a "battle for the soul of this nation", but his message on Thursday night – the message of many of the Democratic speakers this week – was not so different from Harding's.
There was a lot of pressure on Biden to deliver with this speech, particularly when Republicans have suggested the 77-year-old was in decline or "diminished".
At least for one night, the former vice-president, who has given rousing stemwinders in the past, hit all his marks. He was angry when he had to be, and reassuring when needed to be.
Mr Biden gave a powerful speech, delivered powerfully. If he loses in NovemberRead More – Source